Challenges for wheat doubled haploid (DH) production using anther culture include genotype variability in green plant regeneration and spontaneous chromosome doubling. The frequency of chromosome doubling in our program can vary from 14% to 80%. Caffeine or trifluralin was applied at the start of the induction phase to improve early genome doubling. https://www.bekindtopets.com/top-price-Gemplers-4-mil-Disposable-Nitrile-Gloves-Bucket-of-1000-Gloves-p8887-huge-savings/