The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products and solutions ended up digested and cloned into a pET21a+ vector applying NdeI and XhoI restriction enzymes. Some extent mutation was released by the https://carll776yjt8.blog-gold.com/profile